CATCH-Seq
Large Target Capture Sequencing
CATCH-Seq target enrichment technology from Ubiquity Genomics is a proprietary technology that leverages genomic clones to generate probes corresponding to large, contiguous blocks of a genome. &npsp;This is particularly useful when your research calls for sequencing coding and non-coding regions of a gene, including introns and flanking sequences. Sequencing large target regions including introns make it ideal for identification of structural variants and CNV’s.
CATCH-Seq target enrichment kits are also ideal for studies of DNA methylation, by performing targeted bisulfite sequencing after the target enrichment steps. CATCH-Seq target enrichment technology is compatible with any NGS library or platform. Best of all, since CATCH-Seq technology requires no oligonucleotide design and production costs, custom capture reagents are available at a fraction of the cost of other target enrichment methods.
CATCH-Seq technology provides the following advantages:
Large target regions:Better coverage of the targeted region including repeats
Significantly lower cost than oligo-based probes
Faster turnaround time
Multiplexed capture of samples reduces costs
Publications using CATCH-Seq:
Targeted sequencing of large genomic regions with CATCH-Seq.PLoS One. 2014 Oct 30;9(10):e111756. PMID: 25357200
Regulation of DNA methylation dictates CD4 expression during the development of helper and cytotoxic T cell lineages.
Nat Immunol. 2015 Jul;16(7):746-54. PMID: 26030024
Epigenome-wide association study of fasting blood lipids in the Genetics of Lipid-lowering Drugs and Diet Network study.
Circulation. 2014 Aug 12;130(7):565-72. PMID: 24920721
CATCH-Seq target enrichment kits enable the capture and sequencing of large genomic targets. The comprehensive coverage of CATCH-Seq also enables the detection of structural variants and CNVs.