Custom CATCH-Seq

Custom CATCH-Seq Capture Kits

Large target customizable sequencing compatible with standard and bisulfite converted libraries
CATCH-Seq
 

Ubiquity Genomics provides high-quality CATCH-Seq probes, customized for your genomic regions of interest.  These kits enable targeted enrichment of next-generation sequencing libraries for contiguous blocks of the genome at a fraction of the cost of oligonucleotide pools.  CATCH-Seq probes can be synthesized for any species for which there are mapped genomic clones available (BACs or fosmids).

Kits can target a single locus, capturing ~200kb of the region, including introns, exons, and flanking sequences.  Multiple probe sets can be combined to enlarge the region of interest, or to capture multiple loci at once.  CATCH-Seq Target Enrichment Kits thus provide efficient capture of specific target regions from whole genome NGS libraries.  This technology is also ideal for targeted bisulfite sequencing and studies of broad regions of the genome.

 

CATCH-Seq technology provides the following advantages:

  Large target regions including coding and non-coding space
  Multiple target regions can be targeted with a single kit
  Greater specificity with longer probes (200-300bp)
  Better coverage of the targeted region including repeats
  Significantly lower cost than oligo-based probes
  Bisulfite conversion can be incorporated for DNA methylation studies

CATCH-Seq is feasible in any lab and can be automated.

Simple and fast protocol:
1   Hybridization of library to capture probe
2   Wash Steps
3   Optional bisulfite treatment
4   PCR amplification of library prior to sequencing
Two-day protocol.


See example pricing below for custom projects, check the Project Calculator to estimate costs for your project, or email sales@ubiquitygenomics.com to get a quote.

Custom CATCH-Seq Pricing

Order kits for your lab or have us provide complete sequencing service

1 TARGET/GENE

24 Sample Kit

$56/sample
  • Complete Sequencing Service
  • $239/sample
  • Includes:
  • Library Construction,
  • Capture Kits, 80X Sequencing

500 Sample Kit

$20/sample
  • Complete Sequencing Service
  • $100/sample
  • Includes:
  • Library Construction,
  • Capture Kits, 80X Sequencing

5 TARGETS/GENES

24 Sample Kit

$134/sample
  • Complete Sequencing Service
  • $317/sample
  • Includes:
  • Library Construction,
  • Capture Kits, 80X Sequencing

500 Sample Kit

$48/sample
  • Complete Sequencing Service
  • $146/sample
  • Includes:
  • Library Construction,
  • Capture Kits, 80X Sequencing